Additional file 17: Fig. S17. Analysis of bystander editing in multiplex editing hPSCs isolated using XMAS-TREE. Distribution of bystander edits at target loci in mCherry/GFP double positive and unsorted hPSCs using XMAS-TREE based strategies. Orange indicates target A within the editing window. Light grey indicates bystander A within the editing window. Bystander ratio was computed as the frequency of editing the bystander A divided by the percentage of editing at the target A. P-value given for Student’s t-test comparing bystander ratio in mCherry/GFP double positive versus unsorted cells at an indicated bystander A.