Additional file 1: Figure S1. Assessment of RBM45 association with cytoplasmic stress granules (SGs). HEK293 cells were stained for RBM45 and the SG marker protein G3BP. (a) In untreated cells, the distribution of RBM45 is diffuse and nuclear, while the distribution of G3BP is diffuse and cytoplasmic. (b-g) Treatment with the indicated stressors induced the robust formation of G3BP-positive SGs, which were negative for RBM45. RBM45-positive nuclear stress bodies form in response to each stressor. Arsenite = 1 mM arsenite for 1 h, Heat Shock = 42 °C for 1 h, Serum Dep = serum deprivation for 2 h, CdSO4 = 30 μM cadmium sulfate for 2 h, MTX = 20 μM mitoxantrone for 6 h.
|Date made available||Jun 26 2020|
|Publisher||figshare Academic Research System|