Additional file 1: of Characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803

Figure S1. Electrophoresis Gel displaying the DNA fragments created from RT-PCR of (A) E. coli genes acrA and acrB and (B) of the Synechocystis sp. PCC6803 petB gene. Figure S2. Optical density measurements at 730 nm wavelength every 24 h for 168 h of (A) SD277 and its mutant and complementation derivatives, (B) SD100 and its mutant and complementation derivatives, (C) SD277 and its plasmid addition derivatives, and (D) SD100 and its plasmid addition derivatives. The culture conditions were as follows: illumination was 50 μmol photons m− 2 s− 1, temperature was 30 °C, aeration of filtered air was pumped at a rate of 100 mL/min, and CO2 concentration was the normal atmospheric concentration. Figure S3. Electrophoresis gel displaying the DNA fragments created from PCR of using primers of either end fragments of sll0180 in (A) SD100, SD100 ∆sll0180, SD100 ∆sll0180 pSacrA and (B) SD277, SD277 ∆sll0180, SD277 ∆sll0180 pSacrA. Electrophoresis gel displaying the DNA fragments created from PCR using primers of either end fragments of slr2131 in (C) SD100, SD100 ∆slr2131, SD100 ∆slr2131 pacrB, SD277, SD277 ∆slr2131, and SD277 ∆slr2131 pacrB. Electrophoresis gel displaying the DNA fragments created from PCR of using primers of a region within slr2131 in (D) SD100, SD100 ∆slr2131, SD100 ∆slr2131 pacrB, SD277, SD277 ∆slr2131, and SD277 ∆slr2131 pacrB. (DOCX 6501 kb)