4RWA : Synchrotron structure of the human delta opioid receptor in complex with a bifunctional peptide (PSI community target)

Bryan L. Roth (Contributor)
Raymond C. Stevens (Contributor)
Gustavo Fenalti (Contributor)
Nadia A. Zatsepin (Contributor)
Cecilia Betti (Contributor)
Patrick Giguere (Contributor)
Gye Won Han (Contributor)
Andrii Ishchenko (Contributor)
Wei Liu (Scripps Research Institute) (Contributor)
Karel Guillemyn (Contributor)
Haitao Zhang (Contributor)
Daniel James (Contributor)
Dingjie Wang (Contributor)
Uwe Weierstall (Contributor)
John C.H. Spence (Contributor)
Sébastien Boutet (Contributor)
Marc Messerschmidt (Contributor)
Garth J. Williams (Contributor)
Cornelius Gati (Contributor)
Oleksandr M. Yefanov (Contributor)
Thomas A. White (Contributor)
Dominik Oberthür (Contributor)
Markus Metz (Contributor)
Chun Hong Yoon (Contributor)
Anton Barty (Contributor)
Henry N. Chapman (Contributor)
Shibom Basu (Contributor)
Jesse Coe (Contributor)
Chelsie E. Conrad (Contributor)
Raimund Fromme (Contributor)
Petra Fromme (Contributor)
Dirk Tourwé (Contributor)
Peter W. Schiller (Contributor)
Steven Ballet (Contributor)
Vsevolod Katritch (Contributor)
Vadim Cherezov (Contributor)

Dataset

Description

Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:3.28
Classification:MEMBRANE PROTEIN
Release Date:2015-01-14
Deposition Date:2014-12-01
Revision Date:2015-04-01#2017-06-07#2017-11-22
Molecular Weight:92951.73
Macromolecule Type:Protein
Residue Count:832
Atom Site Count:5933
DOI:10.2210/pdb4rwa/pdb

Abstract:
Bifunctional μ- and δ-opioid receptor (OR) ligands are potential therapeutic alternatives, with diminished side effects, to alkaloid opiate analgesics. We solved the structure of human δ-OR bound to the bifunctional δ-OR antagonist and μ-OR agonist tetrapeptide H-Dmt-Tic-Phe-Phe-NH2 (DIPP-NH2) by serial femtosecond crystallography, revealing a cis-peptide bond between H-Dmt and Tic. The observed receptor-peptide interactions are critical for understanding of the pharmacological profiles of opioid peptides and for development of improved analgesics.

Date made available	2015
Publisher	RCSB-PDB

DOI
10.2210/pdb4rwa/pdb

Access Dataset

https://www.rcsb.org/structure/4RWA

Cite this

Roth, B. L. (Contributor), Stevens, R. C. (Contributor), Fenalti, G. (Contributor), Zatsepin, N. A. (Contributor), Betti, C. (Contributor), Giguere, P. (Contributor), Han, G. W. (Contributor), Ishchenko, A. (Contributor), Liu, W. (Contributor), Guillemyn, K. (Contributor), Zhang, H. (Contributor), James, D. (Contributor), Wang, D. (Contributor), Weierstall, U. (Contributor), Spence, J. C. H. (Contributor), Boutet, S. (Contributor), Messerschmidt, M. (Contributor), Williams, G. J. (Contributor), Gati, C. (Contributor), Yefanov, O. M. (Contributor), White, T. A. (Contributor), Oberthuer, D. (Contributor), Metz, M. (Contributor), Yoon, C. H. (Contributor), Barty, A. (Contributor), Chapman, H. N. (Contributor), Basu, S. (Contributor), Coe, J. (Contributor), Conrad, C. E. (Contributor), Fromme, R. (Contributor), Fromme, P. (Contributor), Tourwé, D. (Contributor), Schiller, P. W. (Contributor), Ballet, S. (Contributor), Katritch, V. (Contributor), Cherezov, V. (Contributor) (2015). 4RWA : Synchrotron structure of the human delta opioid receptor in complex with a bifunctional peptide (PSI community target). RCSB-PDB. 10.2210/pdb4rwa/pdb