4PBU : Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state

  • Haiguang Liu (Contributor)
  • Brenda Reeder (Contributor)
  • Chun Hong Yoon (Contributor)
  • Kenneth R. Beyerlein (Contributor)
  • M. Marvin Seibert (Contributor)
  • Richard Kirian (Contributor)
  • Thomas Moore (Contributor)
  • Lukas Lomb (Contributor)
  • Daniel James (Contributor)
  • Hartawan Laksmono (Contributor)
  • Chelsie E. Conrad (Contributor)
  • Francesco Stellato (Contributor)
  • Matthias Frank (Contributor)
  • Thomas A. White (Contributor)
  • Sébastien Boutet (Contributor)
  • Yulia Pushkar (Contributor)
  • Carl Caleman (Contributor)
  • R. Bruce Doak (Contributor)
  • Ana Moore (Contributor)
  • Lorenzo Galli (Contributor)
  • Shibom Basu (Contributor)
  • Michael J. Bogan (Contributor)
  • Ingo Grotjohann (Contributor)
  • Holger Fleckenstein (Contributor)
  • Shatabdi Roy-Chowdhury (Contributor)
  • Jan Steinbrener (Contributor)
  • Despina Milathianaki (Contributor)
  • Shatabdi Roy-Chowdhury (Contributor)
  • Kevin Schmidt (Contributor)
  • Henry N. Chapman (Contributor)
  • Stefano Marchesini (Contributor)
  • Chelsie E. Conrad (Contributor)
  • S. Hau-Riege (Contributor)
  • Karol Nass (Contributor)
  • Mark S. Hunter (Contributor)
  • Raimund Fromme (Contributor)
  • Raymond Sierra (Contributor)
  • Jay How Yang (Contributor)
  • Sadia Bari (Contributor)
  • Marc Messerschmidt (Contributor)
  • Andrew Aquila (Contributor)
  • Robert L. Shoeman (Contributor)
  • Petra Fromme (Contributor)
  • Stephan Kassemeyer (Contributor)
  • John C. H. Spence (Arizona State University) (Contributor)
  • Christopher Kupitz (Contributor)
  • T. C. Chao (Contributor)
  • Lifen Yan (Contributor)
  • Mengning Liang (Contributor)
  • Anton Barty (Contributor)
  • Danielle E. Cobb (Contributor)
  • Nadia A. Zatsepin (Arizona State University) (Contributor)
  • Uwe Weierstall (Contributor)
  • Petra Fromme (Contributor)
  • Dingjie Wang (Contributor)
  • Kimberly N. Rendek (Contributor)
  • Alexandra Ros (Contributor)
  • Katherine M. Davis (Contributor)
  • Garth J. Williams (Contributor)
  • Andrew V. Martin (Contributor)
  • D. P. Deponte (Contributor)
  • Jesse J. Bergkamp (Contributor)



Experimental Technique/Method:X-RAY DIFFRACTION
Release Date:2014-07-16
Deposition Date:2014-04-13
Revision Date:2014-07-30#2014-08-06#2014-10-01#2015-09-23#2017-09-06#2017-11-22
Molecular Weight:692590.19
Macromolecule Type:Protein
Residue Count:5276
Atom Site Count:48924

Photosynthesis, a process catalysed by plants, algae and cyanobacteria converts sunlight to energy thus sustaining all higher life on Earth. Two large membrane protein complexes, photosystem I and II (PSI and PSII), act in series to catalyse the light-driven reactions in photosynthesis. PSII catalyses the light-driven water splitting process, which maintains the Earth's oxygenic atmosphere. In this process, the oxygen-evolving complex (OEC) of PSII cycles through five states, S0 to S4, in which four electrons are sequentially extracted from the OEC in four light-driven charge-separation events. Here we describe time resolved experiments on PSII nano/microcrystals from Thermosynechococcus elongatus performed with the recently developed technique of serial femtosecond crystallography. Structures have been determined from PSII in the dark S1 state and after double laser excitation (putative S3 state) at 5 and 5.5 Å resolution, respectively. The results provide evidence that PSII undergoes significant conformational changes at the electron acceptor side and at the Mn4CaO5 core of the OEC. These include an elongation of the metal cluster, accompanied by changes in the protein environment, which could allow for binding of the second substrate water molecule between the more distant protruding Mn (referred to as the 'dangler' Mn) and the Mn3CaOx cubane in the S2 to S3 transition, as predicted by spectroscopic and computational studies. This work shows the great potential for time-resolved serial femtosecond crystallography for investigation of catalytic processes in biomolecules.
Date made availableJul 16 2014

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