Description
Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:2.3
Classification:HYDROLASE
Release Date:2014-03-12
Deposition Date:2014-01-27
Revision Date:2014-03-26#2014-04-02
Molecular Weight:104055.56
Macromolecule Type:Protein
Residue Count:966
Atom Site Count:7043
DOI:10.2210/pdb4om9/pdb
Abstract:
Autotransporters (ATs) represent a superfamily of proteins produced by a variety of pathogenic bacteria, which include the pathogenic groups of Escherichia coli (E. coli) associated with gastrointestinal and urinary tract infections. We present the first X-ray structure of the passenger domain from the Plasmid-encoded toxin (Pet) a 100 kDa protein at 2.3 Å resolution which is a cause of acute diarrhea in both developing and industrialized countries. Pet is a cytoskeleton-altering toxin that induces loss of actin stress fibers. While Pet (pdb code: 4OM9) shows only a sequence identity of 50% compared to the closest related protein sequence, extracellular serine protease plasmid (EspP) the structural features of both proteins are conserved. A closer structural look reveals that Pet contains a β-pleaded sheet at the sequence region of residues 181-190, the corresponding structural domain in EspP consists of a coiled loop. Secondary, the Pet passenger domain features a more pronounced beta sheet between residues 135 and 143 compared to the structure of EspP.
Resolution:2.3
Classification:HYDROLASE
Release Date:2014-03-12
Deposition Date:2014-01-27
Revision Date:2014-03-26#2014-04-02
Molecular Weight:104055.56
Macromolecule Type:Protein
Residue Count:966
Atom Site Count:7043
DOI:10.2210/pdb4om9/pdb
Abstract:
Autotransporters (ATs) represent a superfamily of proteins produced by a variety of pathogenic bacteria, which include the pathogenic groups of Escherichia coli (E. coli) associated with gastrointestinal and urinary tract infections. We present the first X-ray structure of the passenger domain from the Plasmid-encoded toxin (Pet) a 100 kDa protein at 2.3 Å resolution which is a cause of acute diarrhea in both developing and industrialized countries. Pet is a cytoskeleton-altering toxin that induces loss of actin stress fibers. While Pet (pdb code: 4OM9) shows only a sequence identity of 50% compared to the closest related protein sequence, extracellular serine protease plasmid (EspP) the structural features of both proteins are conserved. A closer structural look reveals that Pet contains a β-pleaded sheet at the sequence region of residues 181-190, the corresponding structural domain in EspP consists of a coiled loop. Secondary, the Pet passenger domain features a more pronounced beta sheet between residues 135 and 143 compared to the structure of EspP.
| Date made available | 2014 |
|---|---|
| Publisher | RCSB-PDB |