Description
Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:3.2
Classification:SIGNALING PROTEIN
Release Date:2014-03-05
Deposition Date:2014-01-02
Revision Date:2017-08-02#2018-02-14
Molecular Weight:52806.82
Macromolecule Type:Protein
Residue Count:468
Atom Site Count:3419
DOI:10.2210/pdb4o9r/pdb
Abstract:
Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
Resolution:3.2
Classification:SIGNALING PROTEIN
Release Date:2014-03-05
Deposition Date:2014-01-02
Revision Date:2017-08-02#2018-02-14
Molecular Weight:52806.82
Macromolecule Type:Protein
Residue Count:468
Atom Site Count:3419
DOI:10.2210/pdb4o9r/pdb
Abstract:
Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
Date made available | 2014 |
---|---|
Publisher | RCSB-PDB |