4O9R : Human Smoothened Receptor structure in complex with cyclopamine

Chong Wang (Contributor)
Gye Won Han (Contributor)
Vsevolod Katritch (Contributor)
Vadim Cherezov (Contributor)
Raymond C. Stevens (Contributor)
Uwe Weierstall (Contributor)
Daniel James (Contributor)
Thomas A. White (Contributor)
Dingjie Wang (Contributor)
Wei Liu (Scripps Research Institute) (Contributor)
John C.H. Spence (Contributor)
R. Bruce Doak (Contributor)
G. Nelson (Contributor)
Petra Fromme (Contributor)
Raimund Fromme (Contributor)
Ingo Grotjohann (Contributor)
Christopher Kupitz (Contributor)
Nadia A. Zatsepin (Contributor)
Haiguang Liu (Contributor)
Shibom Basu (Contributor)
Daniel Wacker (Contributor)
Gye Won Han (Contributor)
Sébastien Boutet (Contributor)
Marc Messerschmidt (Contributor)
Garth J. Williams (Contributor)
J. E. Koglin (Contributor)
M. Marvin Seibert (Contributor)
Markus Klinker (Contributor)
Cornelius Gati (Contributor)
Robert L. Shoeman (Contributor)
Anton Barty (Contributor)
Henry N. Chapman (Contributor)
Richard Kirian (Contributor)
Kenneth R. Beyerlein (Contributor)
Dianfan Li (Contributor)
Syed T A Shah (Contributor)
Nicole Howe (Contributor)
Martin Caffrey (Contributor)

Dataset

Description

Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:3.2
Classification:SIGNALING PROTEIN
Release Date:2014-03-05
Deposition Date:2014-01-02
Revision Date:2017-08-02#2018-02-14
Molecular Weight:52806.82
Macromolecule Type:Protein
Residue Count:468
Atom Site Count:3419
DOI:10.2210/pdb4o9r/pdb

Abstract:
Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

Date made available	2014
Publisher	RCSB-PDB

DOI
10.2210/pdb4o9r/pdb

Access Dataset

https://www.rcsb.org/structure/4O9R

Cite this

Wang, C. (Contributor), Han, G. W. (Contributor), Katritch, V. (Contributor), Cherezov, V. (Contributor), Stevens, R. C. (Contributor), Weierstall, U. (Contributor), James, D. (Contributor), White, T. A. (Contributor), Wang, D. (Contributor), Liu, W. (Contributor), Spence, J. C. H. (Contributor), Bruce Doak, D. R. (Contributor), Nelson, G. (Contributor), Fromme, P. (Contributor), Fromme, R. (Contributor), Grotjohann, I. (Contributor), Kupitz, C. (Contributor), Zatsepin, N. A. (Contributor), Liu, H. (Contributor), Basu, S. (Contributor), Wacker, D. (Contributor), Won Han, G. (Contributor), Boutet, S. (Contributor), Messerschmidt, M. (Contributor), Williams, G. J. (Contributor), Koglin, J. E. (Contributor), Marvin Seibert, S. M. (Contributor), Klinker, M. (Contributor), Gati, C. (Contributor), Shoeman, R. L. (Contributor), Barty, A. (Contributor), Chapman, H. N. (Contributor), Kirian, R. A. (Contributor), Beyerlein, K. R. (Contributor), Li, D. (Contributor), Shah, S. T. A. (Contributor), Howe, N. (Contributor), Caffrey, M. (Contributor) (2014). 4O9R : Human Smoothened Receptor structure in complex with cyclopamine. RCSB-PDB. 10.2210/pdb4o9r/pdb