2NDJ : Structural Basis for KCNE3 and Estrogen Modulation of the KCNQ1 Channel

  • Jens Meiler (Contributor)
  • Brett M. Kroncke (Contributor)
  • Richard C. Welch (Contributor)
  • Jarrod Smith (Contributor)
  • Congbao Kang (Contributor)
  • Nicholas J. Sisco (Contributor)
  • Wade Van Horn (Contributor)
  • David P. Nannemann (Contributor)
  • Alfred L. George (Contributor)
  • Yuanli Song (Contributor)
  • Charles R. Sanders (Contributor)
  • Carlos G. Vanoye (Contributor)

Dataset

Description

Experimental Technique/Method:SOLUTION NMR
Resolution:
Classification:MEMBRANE PROTEIN
Release Date:2016-09-21
Deposition Date:2016-06-09
Revision Date:2017-12-20
Molecular Weight:12799.58
Macromolecule Type:Protein
Residue Count:112
Atom Site Count:823
DOI:10.2210/pdb2ndj/pdb

Abstract:
The single-span membrane protein KCNE3 modulates a variety of voltage-gated ion channels in diverse biological contexts. In epithelial cells, KCNE3 regulates the function of the KCNQ1 potassium ion (K(+)) channel to enable K(+) recycling coupled to transepithelial chloride ion (Cl(-)) secretion, a physiologically critical cellular transport process in various organs and whose malfunction causes diseases, such as cystic fibrosis (CF), cholera, and pulmonary edema. Structural, computational, biochemical, and electrophysiological studies lead to an atomically explicit integrative structural model of the KCNE3-KCNQ1 complex that explains how KCNE3 induces the constitutive activation of KCNQ1 channel activity, a crucial component in K(+) recycling. Central to this mechanism are direct interactions of KCNE3 residues at both ends of its transmembrane domain with residues on the intra- and extracellular ends of the KCNQ1 voltage-sensing domain S4 helix. These interactions appear to stabilize the activated "up" state configuration of S4, a prerequisite for full opening of the KCNQ1 channel gate. In addition, the integrative structural model was used to guide electrophysiological studies that illuminate the molecular basis for how estrogen exacerbates CF lung disease in female patients, a phenomenon known as the "CF gender gap."
Date made availableSep 21 2016
PublisherRCSB-PDB

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